Effectiveness of Antagonist Fungi (Trichoderma sp., Gliocladium sp., and Verticillium sp.) against Sclerotium Rolfsii Causes Damping-Off Disease on Soybean Glycine Max L. Merill.

Muhamad Kindi, Ika Rochdjatun Sastrahidayat, Anton Muhibuddin, Hunsa Punnapayak

Abstract


Efforts to increasing soybean production is inseparable from the various constraints. One of them caused by plant disease like soil. Soil borne diseases is one of the factor that reduce leguminous plants production. The leguminous plants attacked by soil born disease which is known as S. rolfsii. One of the alternative control isthe use of biological agents. Bilogical agents that used in this research were Trichoderma sp., Gliolcadium sp., and Verticillium sp. The pusrpose of this research were to know the effectiveness of Trichoderma sp., Gliolcadium sp., Verticillium sp. against S. rolfsii. The research was conducted 3 tests, all three tests used completly randomized design and if there is siginificant difference, continuedwith LSD test. The first test was direct oposition test, consist of 4 treatments and 5 replications. The second test was steam culture test, consist of 4 treatments and 5 replications. The third test was antagonist test in the glass house that consist of 4 treatments and 6 replications. The result of this research in direct oposition test on 7 days after inoculation, which is Trichoderma sp., can inhibit the pathogen by 61,71%, Gliocladium sp. 59,74% and Verticillium sp. 34%. On the steam culture method test Trichoderma sp., Gliocladium sp., and Verticillium sp., can inhibit the pathogen with the pathogen colony diameter by 3,70 cm, 3,24 cm dan 6,82 cm. The third test was antagonist test in the glass house, and the results were attack percentage with treatment Trichoderma sp., 51,67%, Gliocladium sp. 52,83%, Verticillium sp. 91%, Control (without antagonist) 98%.


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